To exert the imbibition between cracks and matrix effectively and enhance the development effect of tight oil reservoirs, a physical simulation method for imbibition in different scales of cores is developed by combining a high-pressure large-model physical simulation system and nuclear magnetic resonance technology (NMR) to investigate the influencing factors of imbibition process in tight reservoirs, and construct a quantitative evaluation method for the imbibition in water flooding. The results show that in the process of counter-current imbibition, the lower the permeability, the later the oil droplet precipitation, the longer the imbibition equilibrium time, and the lower the recovery degree. Fractures can effectively expand the area of imbibition and the front edge of imbibition in the contact between the dense matrix and water, reduce the resistance of oil discharge, and improve the imbibition speed and the degree of recovery. The more hydrophilic the rock, the higher the imbibition rate and imbibition recovery of tight rocks. In the process of co-current imbibition, the lower the permeability, the more obvious the imbibition, and the displacement recovery is positively correlated with permeability, while the imbibition recovery is negatively correlated with the permeability. It also shows that the imbibition distance of the cyclic water injection is greater than that of the counter-current imbibition, and the higher the permeability and the injection multiple, the longer the imbibition distance. The combination of large-scale volume fracturing with changing reservoir wettability and cyclic water injection is conducive to improving the imbibition ability of tight reservoirs.